Method for replica-plating Neurospora
نویسندگان
چکیده
منابع مشابه
A Plating Method for Genetic Analysis in Neurospora.
N Neurospora crassa, as in other ascomycetes with a spreading type of I growth, it has been necessary to obtain genetic data by the laborious method of isolating single ascospores in test tubes, and, in the case of biochemical mutants, transferring each of the cultures thus obtained to various media in order to characterize it (BEADLE 1945). The discovery that sorbose and certain other agents w...
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409 repeated centrifugation and/or a series of sieving steps N0rgaard described a technique utilizing density gradient centrifugation. Although high purity and ultrastructural preservation were seen, the procedure required renal perfusion with a special medium, and yields of pure glomeruli were relatively low (10 to 20%). The current procedure is simple and rapid and requires neither renal perf...
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and spread during the summer to widely separated locations. In addition to white perch (Roccus americanus), striped bass (R. saxatilis) were involved to a lesser degree. Carefully selected moribund specimens were collected and delivered to the laboratory. Microscopy disclosed an abundance of polarly staining gram-negative rods in blood and organs of diseased fish. First isolations of these bact...
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Rhizosphere investigations are restricted by the sheer number of cultures that must be handled in the multitude of different tubed media needed to characterize each isolate. A current rhizosphere study called for the use of 25 different diagnostic media with each of hundreds of isolates, a burdensome and laborious problem. Moreover, the replica plating technique of J. Lederberg and E. M. Lederb...
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The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 x 10(-4)/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. kanamycin or neomycin. Transformed primary colonies were easily recognized due to a different colon...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1994
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1389